3 nyob rau hauv 1 Lub raum Kev Ua Haujlwm Kev Xeem Strip siv lub sijhawm kawg-kawg txoj kev los ntsuas Uric Acid (UA), Creatinine (CR) thiab Urea (UR) concentrations hauv cov ntshav, ntshav lossis ntshav. Lub kaw lus saib xyuas qhov kev hloov pauv ntawm qhov nqus ntawm 620 nm ntawm lub sijhawm ncua sijhawm. Kev hloov pauv hauv absorbance yog ncaj qha proportional rau qhov concentration ntawm metabolite hauv cov qauv.
UA: Hauv cov tshuaj tiv thaiv, uric acid yog oxidized rau allantoin thiab hydrogen peroxide los ntawm uricase.
Peroxidase catalyzes cov tshuaj tiv thaiv ntawm hydrogen peroxide nrog 4-aminoantipyrine thiab phenol rau
tsim cov khoom xim quinoneimine. Qhov kev hloov hauv absorbance yog ncaj qha proportional rau qhov concentration ntawm uric acid nyob rau hauv cov qauv.
CR: Creatinine hauv cov qauv yog hydrolyzed rau creatine los ntawm kev ua ntawm creatininase. Ib ntu ntawm peb kauj ruam enzymatic ua ke siv creatinase, sarcosine oxidase, thiab peroxidase ua rau oxidative coupling ntawm 4-aminoantipyrine los ua cov xim xiav.
UR: Urea hauv cov qauv yog hydrolyzed rau NH 3 thiab CO 2 los ntawm kev ua ntawm urease, thiab cov txheej txheem qhia uas muaj qhov taw qhia uas ua rau muaj kev hloov pauv los ntawm gaseous ammonia.
